Lipase is an enzyme that catalyses the hydrolysis of lipids. An experiment was carried out to investigate changes in pH when lipase is added to a food sample containing a high proportion of lipids.
The results are shown in the graph.
Which statements are possible explanations of the results of the experiment between 50 minutes and 60 minutes?
1) Enzyme concentration becomes the limiting factor.
2) Substrate concentration becomes the limiting factor.
3) All the enzyme active sites are occupied.
4) Denaturation of the enzyme by the products takes place.
5) Products are acting as competitive inhibitors.
1 )
1, 2 and 3
2 )
1, 4 and
3 )
2, 3 and 4
2, 4 and 5
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