Which steps are needed to find the actual width of a xylem vessel viewed in transverse section using a ×40 objective lens?
1) Convert from mm to μm by multiplying by 10–3.
2) Calibrate the eyepiece graticule using a stage micrometer on ×10 objective lens.
3) Measure the width of the xylem vessel using an eyepiece graticule.
4) Multiply the number of eyepiece graticule units by the calibration of the eyepiece graticule.
1 )
1, 2, 3 and 4
2 )
2, 3 and 4 only
3 )
1 and 2 only
3 and 4 only
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