In an investigation, the same concentration of the enzyme phosphorylase was added to different concentrations of glucose phosphate and incubated at ${30^ \circ }C$. At 1 minute intervals, one drop of the reaction mixture was removed and added to a drop of iodine solution on a white tile.
The diagram shows the results of this investigation.
What explains the trend in the results of this investigation?
1 )
Phosphorylase catalyses a reaction converting glucose phosphate to starch.
2 )
The maximum rate of reaction is reached at $20{\text{ }}mg{\text{ }}d{m^{-3}}$ of glucose phosphate.
Substrate concentration is limiting at concentrations of glucose phosphate $25{\text{ }}mg{\text{ }}d{m^{-3}}$ or less.
4 )
Enzyme concentration is limiting at concentrations of glucose phosphate $25{\text{ }}mg{\text{ }}d{m^{-3}}$ or less.
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