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An investigation was carried out on the effect of temperature on an enzyme-catalysed reaction.

The enzyme and its substrate were initially placed into separate test-tubes and raised to the temperature required. They were then mixed and placed into four tubes A, B, C and D.

These tubes were incubated for the time and at the temperature stated. The mass of the product formed was then measured.

In which tube was the rate of reaction highest? 

1 ) 

A

2 ) 

B

3 ) 

C

4 ) 

D

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