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High concentrations of ethanol can disrupt cell membrane structure and make the cell surface membrane leaky.

Yeast cells release ethanol as a waste product of metabolism.

Yeast cells can alter the composition of the cell surface membrane to reduce the effect of ethanol.

● A saturated fatty acid, X, is replaced by an unsaturated fatty acid, Y.
● Y has a shorter hydrocarbon chain than X.
● There is an increase in the phospholipid to protein ratio.

Which statement would explain why the effect of ethanol is reduced?

1 ) 

A greater proportion of phospholipid may increase the entry of ethanol via channel proteins.

2 ) 

An increase in hydrophobic interaction by phospholipid tails improves membrane stability.

3 ) 

An increase in the proportion of fatty acids with double bonds decreases membrane fluidity.

4 ) 

The shorter hydrocarbon chain of Y decreases membrane fluidity.

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