Catechol is a chemical found in a number of fruits. Catechol can be oxidised to a quinone by the enzyme catechol oxidase.
Catechol oxidase is inhibited by parahydroxybenzoic acid (PHBA), which is structurally similar to catechol. In the presence of PHBA, the Michaelis-Menten constant (Km) for catechol oxidase increases.
How does PHBA inhibit catechol oxidase?
1) PHBA and catechol can both bind to the enzyme but not at the same time.
2) PHBA is a non-competitive inhibitor of catechol oxidase.
3) PHBA decreases the Vmax of the reaction.
1 )
1, 2 and 3
1 only
3 )
2 only
4 )
3 only
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