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An investigation was carried out into the effect of different treatments on the permeability of the cell surface membranes and tonoplasts of beetroot cells. Beetroot cell vacuoles contain a red pigment. This pigment is unable to pass out of the cells because it cannot diffuse through the tonoplasts or cell surface membranes.

$1c{m^3}$ cubes were cut from beetroot tissue and washed in running water for 20 minutes to remove any pigment released from damaged cells.

The cubes were then placed in test-tubes with different contents and observed for five minutes.

Which row shows a correct explanation for the observation recorded for one of the treatments?

1 ) 

A

2 ) 

B

3 ) 

C

4 ) 

D

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