What occurs during protein denaturation by extremes of pH?
1 breakage of peptide bonds leading to loss of shape of active site
2 disruption of existing ionic bonds between amino acid R-groups
3 loss of $\alpha $-helical regular arrangement of amino acids
4 loss of protein tertiary structure resulting in loss of function
1 )
1, 2, 3 and 4
2, 3 and 4 only
3 )
1 and 3 only
4 )
2 and 4 only
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