Monoclonal antibodies are produced for use in diagnosis or treatment of disease. To obtain the antibodies for an antigen, a mouse is injected with the antigen.
Some of the events in the production of the monoclonal antibody are listed.
1 Plasma cells are fused with cancer cells to form a hybridoma.
2 Hybridomas that secrete the required antibody are identified and cloned.
3 B-lymphocytes that recognise the antigen multiply and become plasma cells.
4 Hybridomas divide by mitosis and secrete antibodies.
5 Plasma cells are removed from the mouse spleen.
What is the sequence of the first four events in the production of the monoclonal antibody?
1 )
$3 \to 5 \to 1 \to 2$
$3 \to 5 \to 1 \to 4$
3 )
$5 \to 1 \to 2 \to 4$
4 )
$5 \to 1 \to 4 \to 2$
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