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The graph shows the effect of temperature on the rate at which the enzyme in a biological washing powder digests and removes fruit juice stains. 

Which statements explain the shape of the graph at temperatures higher than X?

1 Bonds are broken between the R groups of the amino acids in the polypeptide chains of the enzyme.

2 There are more collisions between the enzyme and its substrate.

3 The tertiary structure of the enzyme is altered.

4 The shapes of the active site and the substrate are no longer complementary. 

1 ) 

1, 2 and 3 

2 ) 

1, 2 and 4 

3 ) 

1, 3 and 4

4 ) 

2, 3 and 4

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