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An experiment was conducted to investigate the effect of temperature on the activity of the enzyme $\beta $-glucosidase. The enzyme was tested when in solution (free) and when immobilised in alginate beads.

The results are shown in the graph below.  

Which statement about the effect of immobilisation of $\beta $-glucosidase is correct?  

1 ) 

It increases the kinetic energy of the enzyme. 

2 ) 

It inhibits the activity of the enzyme.  

3 ) 

It reduces the optimum temperature of the enzyme. 

4 ) 

It stabilises the enzyme against denaturation. 

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