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An investigation into the rate of an enzyme-catalysed reaction was carried out. During the investigation the concentration of the substrate was kept higher than the concentration of the enzyme.

During this investigation, which change in the variables would always lead to an increase in the rate of the reaction?

1 increase in enzyme concentration

2 increase in pH

3 increase in temperature  

1 ) 

1 and 2

2 ) 

1 and 3

3 ) 

2 and 3 

4 ) 

1 only

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