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DNA replication in bacteria was investigated. Bacteria were grown in a medium with only heavy
nitrogen, ${}^{15}N$, until all of the bacterial DNA was heavy.

These bacteria were moved from the heavy nitrogen medium and cultured in a medium with only
light nitrogen, ${}^{14}N$. This formed the first generation.

The bacteria continued to reproduce, and a sample of bacteria was collected from the second
generation and the DNA analysed.

Hybrid DNA contains heavy and light DNA.

Which row is correct for the second generation?

1 ) 

A

2 ) 

B

3 ) 

C

4 ) 

D

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