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In order to estimate the quantity of glucose in a solution, equal volumes of a range of known concentrations were mixed with equal excess volumes of Benedict’s solution and placed in a thermostatically controlled water-bath at ${90^ \circ }C$ for the same length of time.

The unknown solution was then treated in the same way and the colours of the known and unknown solutions compared.

What is the independent variable in this procedure? 

1 ) 

concentration of glucose

2 ) 

final colour of solutions

3 ) 

temperature of water-bath

4 ) 

volumes of glucose solutions

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