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The test for non-reducing sugars requires a second Benedict’s test to be carried out. Which set of steps is the correct method for carrying out the non-reducing sugar test before carrying out the second Benedict’s test? 

1 ) 

Perform the Benedict’s test, which gives a negative result. Neutralise with sodium hydrogencarbonate. Warm gently with dilute hydrochloric acid. 

2 ) 

Perform the Benedict’s test, with the colour of the solution remaining red. Boil with dilute hydrochloric acid. Neutralise with sodium hydrogencarbonate. 

3 ) 

Perform the Benedict’s test, with the colour of the solution remaining blue. Boil with dilute hydrochloric acid. Neutralise with sodium hydrogencarbonate. 

4 ) 

Perform the Benedict’s test, which gives a negative result. Neutralise with sodium hydrogencarbonate. Warm gently with dilute hydrochloric acid. 

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