The test for non-reducing sugars requires a second Benedict’s test to be carried out. Which set of steps is the correct method for carrying out the non-reducing sugar test before carrying out the second Benedict’s test?
1 )
Perform the Benedict’s test, which gives a negative result. Neutralise with sodium hydrogencarbonate. Warm gently with dilute hydrochloric acid.
2 )
Perform the Benedict’s test, with the colour of the solution remaining red. Boil with dilute hydrochloric acid. Neutralise with sodium hydrogencarbonate.
Perform the Benedict’s test, with the colour of the solution remaining blue. Boil with dilute hydrochloric acid. Neutralise with sodium hydrogencarbonate.
4 )
Perform the Benedict’s test, which gives a negative result. Neutralise with sodium hydrogencarbonate. Warm gently with dilute hydrochloric acid.
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