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A transcription error results in the deletion of one nucleotide from the middle of a primary transcript. mRNA forms from the primary transcript.

Which statement describes one possible effect of this deletion on the protein translated from this mRNA? 

1 ) 

The protein will be unchanged as the same amino acids can be coded by another codon formed by the deletion. 

2 ) 

The tertiary structure of the protein is not affected as only one amino acid has been changed by the deletion.

3 ) 

Only one amino acid has been changed by the deletion but this changes the quaternary structure of the protein. 

4 ) 

The sequence of amino acids in the protein will be different as all the codons from the deletion onwards are changed. 

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