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The diagram shows the mass of product formed over time in three reactions using the same substrate and enzyme.

The volumes of substrate, enzyme and temperature were kept constant in each reaction.

Which statement explains the difference in these reactions?

1 ) 

The pH in reactions 2 and 3 has denatured the enzyme.

2 ) 

There is a non-competitive inhibitor present in reaction 3.

3 ) 

There is the highest concentration of enzyme in reaction 1.

4 ) 

There is the highest concentration of substrate in reaction 1.

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