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In an experiment, fluorescent dyes were used to label proteins on the outer surface of cell surface membranes. Living human cells were labelled with one colour of fluorescent dye and mouse cells with a different colour of fluorescent dye.

A human cell and a mouse cell are then fused to form a hybrid cell.

At first, the different fluorescent labels remain separate, but after 40 minutes they are distributed randomly across the hybrid cell surface membrane.

What does this experiment show?

1 ) 

Proteins are found only on the outer surface of cell surface membranes.  

2 ) 

Proteins in the outer layer of a bilayer do not penetrate into the inner layer.  

3 ) 

Proteins move freely in the phospholipids of a bilayer. 

4 ) 

The cell surface membranes of the two cells are bilayers.  

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