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An experiment was carried out in which the enzyme lipase was used to hydrolyse a triglyceride.

The pH was recorded at regular intervals during the reaction.

The results are shown in the table.

At 14 minutes unreacted triglyceride was still present.

What explains the results after 10 minutes?

1 ) 

The end-products acted as competitive inhibitors.

2 ) 

The end-products acted as non-competitive inhibitors.

3 ) 

The enzyme reaction had reached Vmax.

4 ) 

The tertiary structure of the enzyme had been lost.

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