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Yeast contains the enzyme catalase which catalyses the breakdown of hydrogen peroxide $\left( {{H_2}{O_2}} \right)$ as shown.  

Yeast was added to a solution of hydrogen peroxide and the total volume of oxygen released was
recorded every 30 seconds for 2 minutes. All other variables were standardised.

The data is shown in the table.  

What explains the pattern of the data?

1 ) 

The rate of reaction increases as more enzyme–substrate complexes are formed. 

2 ) 

The rate of reaction increases as the enzyme reaches its maximum velocity $\left( {{V_{max}}} \right)$. 

3 ) 

The volume of oxygen released decreases as the enzymes begin to denature. 

4 ) 

The volume of oxygen released decreases as more substrate is converted into product. 

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