An experiment was carried out to compare the effect of pH on the activity of an enzyme that was in solution and the same enzyme that had been immobilised on a gel. All other variables were kept the same.
The results are shown in the graph.
Which statement explains these results when the enzyme is immobilised?

1 )
The primary structure has changed.
2 )
The secondary structure has changed.
The tertiary structure has changed.
4 )
The quaternary structure has changed.
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