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An experiment was carried out to compare the effect of pH on the activity of an enzyme that was in solution and the same enzyme that had been immobilised on a gel. All other variables were kept the same.

The results are shown in the graph.

Which statement explains these results when the enzyme is immobilised?

1 ) 

The primary structure has changed.

2 ) 

The secondary structure has changed.

3 ) 

The tertiary structure has changed.

4 ) 

The quaternary structure has changed.

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