Some molecules fluoresce when illuminated with ultraviolet light. Changes in the structure or shape of such molecules can change the intensity of the fluorescence.
The enzyme tryptophan synthase fluoresces bluish-green because of a phosphate group associated with the active site. The enzyme catalyses the reaction shown.
serine + indole $\to$ tryptophan
Adding serine to the enzyme increases the intensity of the fluorescence, but when indole is also
added the bluish-green fluorescence decreases in intensity.
What may be concluded from these observations?
1- Serine and indole attach to the active site of the enzyme.
2- An enzyme–serine complex is formed.
3- An enzyme–serine–indole complex is formed.
1, 2 and 3
2 )
1 and 2 only
3 )
1 and 3 only
4 )
2 and 3 only
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