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An antibiotic enters bacterial cells through a membrane channel protein, P.

Some bacterial cells have shown resistance to this antibiotic by acquiring a mutation which alters P. This mutation prevents the entry of the antibiotic into the cell.

Which conclusions can be drawn about how resistance to this antibiotic developed in these bacteria?

1 The mutation changed the order of the amino acids in the gene coding for P.

2 The mutation resulted in the production of P with an altered tertiary structure.

3 The antibiotic is a hydrophobic molecule and so cannot cross the phospholipid bilayer to enter the cell.  

1 ) 

1, 2 and 3 

2 ) 

1 and 3 only

3 ) 

2 and 3 only

4 ) 

2 only

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