A student carried out experiments to investigate the effect of enzyme concentration on the rate of hydrolysis (break down) of protein in milk.
When the enzyme and milk were mixed, the protein was hydrolysed and the mixture changed from cloudy to clear.
The student investigated five different enzyme concentrations and recorded the time taken to reach the end-point for each.
What is an appropriate control for this investigation?
Carrying out a further experiment where the enzyme solution is replaced with water.
2 )
Carrying out each experiment in a thermostatically regulated water-bath at ${35^ \circ }C$.
3 )
Performing three repeat experiments for each of the five enzyme concentrations.
4 )
Using the same volume of enzyme solution for each of the five experiments.
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