Biology 9700/33 Oct Nov 2022

- Enzyme E, coagulation of milk, hydrolysis of peptide bonds, calcium ions, temperature effect.
- Experimental setup, water-bath temperatures, independent variable identification, control suggestions.
- Concentration of milk, modification of procedure, time to reach end-point, enzyme activity measurement.
- Graph plotting, enzyme concentration activity correlation, trend analysis, explanation of results.
- Observable differences in plant stem sections, vascular bundles counting, sampling techniques, advantages of multiple vascular bundles.
- Safety precautions, use of protective equipment, handling of harmful substances, appropriate units in measurements.