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Lipase is a digestive enzyme produced by the pancreas that catalyses the hydrolysis of dietary lipids. The table shows how the pH of a liquid food containing a high proportion of lipids decreases over time. 

Which statements are possible explanations of the results of the experiment between 50 and 60 minutes?

1) Enzyme concentration becomes the limiting factor.
2) Substrate concentration becomes the limiting factor.
3) All the enzyme active sites are saturated.
4) Denaturation of the enzyme by the products.
5) Products are acting as competitive inhibitors.

1 ) 

1, 2 and 3

2 ) 

1, 4 and 5

3 ) 

2, 3 and 4

4 ) 

2, 4 and 5

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